Glacios: X-FEG and Falcon4

A TFS Glacios 200kV instrument with X-FEG and FalconIV

The 200kV Glacios with X-FEG and Falcon IV detector is a high-resolution cryo-EM instrument that is designed for resolution and throughput. Its 12-grid autoloader allows efficient loading and unloading of cryo-EM grids. The automated SerialEM and EPU software systems are used to screen grids for suitability for high-resolution analysis. The Glacios itself can determine structures are better than 2.0Å resolution in favorable cases.

Instrument Parameters:

Accelerating Voltage 200 kV
Electron Source X-FEG
Stage Tilt Range ± 70°
C2 Apertures 150, 100, 70 and 50 µm
Objective Apertures 100, 70 and 30 µm
Operating Temperature ~80 K
Information Limit at 0° / ±70° tilt 0.23 nm / 0.34 nm
Cs 2.7 mm
Number of cartridge positions for grids 12 total
Sample exchange time ~3 mins from autoloader cassette to column
Camera Falcon4
Camera pixels 14 micrometer pixel size, 4096x4096 pixels, Electron Event Recording capable
Camera Performance DQE (1/2 Nyquist) 0.70
Camera Performance DQE (Nyquist) 0.28
Acquisition software SerialEM, EPU and TFS Tomography