The Titan Krios G4 with Cold-FEG and Falcon IV detector: A powerful instrument for single particle Cryo-EM at ultra-high resolution
This instrument is equipped as the one above, but without the energy filter.
Titan #2: Cold-FEG, Falcon4
A 300kV TFS Titan Krios G4 with E-CFEG and FalconIV
Instrument Parameters:
| Accelerating Voltage | 80 ... 300 kV |
| Electron Source | E-CFEG (Cold FEG) |
| Stage Tilt Range | ± 70° |
| C1 Apertures | 2000, 70, 50 and 30 µm |
| C2 Apertures | 150, 100, 50 and 20 µm |
| Objective Apertures | 100, 70 and 30 µm |
| Operating Temperature | ~80 K |
| Information Limit at 300kV at 0° / ±70° tilt | 0.12 nm / 0.23 nm |
| Cs | 2.7 mm |
| Number of cartridge positions for grids | 12 total |
| Sample exchange time | ~3 mins from autoloader cassette to column |
| Camera | Falcon4 |
| Camera pixels | 14 micrometer pixel size, 4096x4096 pixels, Electron Event Recording capable |
| Camera Performance DQE (1/2 Nyquist) | 0.70 |
| Camera Performance DQE (Nyquist) | 0.28 |
| Acquisition software | SerialEM, EPU and TFS Tomography |