Titan #1: Cold-FEG, SelectrisX, Falcon4

A 300kV TFS Titan Krios G4 with E-CFEG, SelectrisX, and FalconIV

The Titan Krios G4 with Cold-FEG, SelectrisX energy filter, and Falcon IV detector: Solve structures at record-breaking resolutions

The 300kV Titan Krios G4 Cryo-TEM with the Thermo Scientific E-CFEG and the groundbreaking Thermo Scientific Selectris X Imaging Filters to advance cryo-EM discovery with unprecedented resolution, speed, and ease of use.

The E-CFEG is a cold field emission gun with a narrow energy spread that results in higher contrast images and enables atomic resolution. This add-on makes it possible to efficiently solve structures at resolutions of 2.0 Å or less.

Selectris X Imaging Filters provide unique stability, accessibility, and performance. Paired with the Thermo Scientific Falcon 4 Direct Electron Detector, these two add-ons produce better quality images up to 10 times faster than the previous generation.

The group of Sjors Scheres together with scientists from FEI/TFS recently published an article in Nature, describing the use of a prototype instrument in this configuration to study protein structures at never-before-achieved resolutions, obtaining a 1.2 Å resolution structure of the iron-storing protein apoferritin and a 1.7 Å resolution of the GABA type A receptor-associated protein, with an even sharper resolution in key parts of the protein.

Instrument Parameters:

Accelerating Voltage 80 ... 300 kV
Electron Source E-CFEG (Cold FEG)
Stage Tilt Range ± 70°
C1 Apertures 2000, 70, 50 and 30 µm
C2 Apertures 150, 100, 50 and 20 µm
Objective Apertures 100, 70 and 30 µm
Operating Temperature ~80 K
Information Limit at 300kV at 0° / ±70° tilt 0.12 nm / 0.23 nm
Cs 2.7 mm
Number of cartridge positions for grids 12 total
Sample exchange time ~3 mins from autoloader cassette to column
Imaging filter and detector SelectrisX
Camera Falcon4
Camera pixels 14 micrometer pixel size, 4096x4096 pixels, Electron Event Recording capable
Camera Performance DQE (1/2 Nyquist) 0.70
Camera Performance DQE (Nyquist) 0.28
Acquisition software SerialEM, EPU and TFS Tomography