Leica EM GP

Automatic Plunge Freezing with Bare Grid Technique Leica EM GP

CONFIGURATION

Automated rapid-immersion freezing device for the vitrification of biological samples for cryo EM. The reliability and reproducibility allows the preparation of frozen hydrated specimens for transmission electron microscopy under cryo conditions with repeatable experimental results.

APPLICATION

The Leica EM GP plunge freezes samples into liquid ethane after removing excess fluid by automatic blotting. This is the bare grid technique. Prior to freezing, the sample is maintained in a temperature and humidity controlled environmental chamber, adjustable between +4°C and +60°C and room humidity to 99 %.

Optimally suited for rapid freeze preservation and immobilization of single particle, macromolecules, filamentous and crystalline structures. Can be applied to a range of samples sizes, as small as a few hundred kDa to the size of whole bacteria or cultured cell preparations of thin cells such as neurons.