Philippe Ringler

Current Project

Structural analysis of proteins by transmission electron microscopy (TEM). In particular, TEM analysis of antibody constructs and conjugates, epitope mapping, conformation and homogeneity analysis.

Publications

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Protease-activation using anti-idiotypic masks enables tumor specificity of a folate receptor 1-T cell bispecific antibody

M. Geiger, K.-G. Stubenrauch, J. Sam, W.F. Richter, G. Jordan, J. Eckmann, C. Hage, V. Nicolini, A. Freimoser-Grundschober, M. Ritter, M.E. Lauer, Stahlberg, H., Ringler, P., J. Patel, E. Sullivan, S. Grau-Richards, S. Endres, S. Kobold, P. Umaña, P. Brünker, C. Klein

Nature Commun. 11(1), 3196 (2020)

DOI: DOI

22

The Contorsbody, an antibody format for agonism: design, structure, and function

Georges, G.J., Dengl, S., Bujotzek, A., Hesse, F., Fischer, J.A.a., Gärtner, A., Benz, J., Lauer, M.E., Ringler, P., Stahlberg, H., Plath, F., Brinkmann, U., Imhof-Jung, S.

Comp. Struct. Biol. J. 18, 1210-1220 (2020)

DOI: DOI

21

High-resolution cryo-EM structure of urease from the pathogen Yersinia enterocolitica

Righetto, R., Anton, L., Rima, L., Jakob, R.P., Zivanov, J., Mahi, M.-A., Ringler, P., Schwede, T., Maier, T., Stahlberg, H.

Nature Commun. 11, 5101 (2020)

DOI: Nature Comm.

20

DuoMab: a novel CrossMab-based IgG-derived antibody format for enhanced antibody-dependent cell-mediated cytotoxicity

Sustmann, C., Dickopf, S., Regula, J.T., Kettenberger, H., Mølhøj, M., Gassner, C., Weininger, D., Fenn, S., Manigold, T., Kling, L., Künkele, K.P., Schwaiger, M., Bossenmaier, B., Griese, J.J., Hopfner, K.P., Graff, A., Stahlberg, H., Ringler, P., Lauer, M.E., Brinkmann, U., Schaefer, W., Klein, C.

MAbs 11(8), 1402-1414 (2019)

PubMed

DOI: DOI

19

Two new polymorphic structures of alpha-synuclein solved by cryo-electron microscopy

Guerrero-Ferreira, R., Taylor, N., Arteni, A.-A., Kumari, P., Mona, D., Ringler, P., Britschgi, M., Lauer, M.E., Verasdock, J., Riek, R., Melki, R., Meier, B.H., Böckmann, A., Bousset, L., Stahlberg, H.

eLife 8:e48907, (2019)

PubMed

DOI: DOI

18

Tumor-targeted 4-1BB agonists for combination with T cell bispecific antibodies as off-the-shelf therapy

Claus, C., Ferrara, C., Xu, W., Sam, J., Lang, S., Uhlenbrock, F., Albrecht, R., Herter, S., Schlenker, R., Hüsser, T., Diggelmann, S., Challier, J., Mössner, E., Hosse, RJ., Hofer, T., Brünker, P., Joseph, C., Benz, J., Ringler, P., Stahlberg, H., Lauer, M., et al., Klein, C., Umana, P.

Science Translational Medicine 11(496), 1-12 (2019)

PubMed

DOI: DOI

17

Cryo-EM structure of alpha-synuclein fibrils

Guerrero-Ferreira, R., Taylor, N., Mona, D., Ringler, P., Lauer, M.E., Riek, R., Britschgi, M., Stahlberg, H.

eLife 7(e36402), (2018)

PubMed

DOI: DOI

16

Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene

Seuring, C., Ayyer, Filippaki, Barthelmess, Longchamp, Ringler, P., Pardini, Wojtas, Coleman, Dörner, Fuglerud, Hammarin, Habenstrein, Langkilde, Loquet, Meents, Riek, Stahlberg, H., Boutet, Hunter, Koglin, Liang, Ginn, Millane, Frank, Barty, Chapman

Nature Communications 9(1), 1836 (2018)

PubMed

DOI: DOI

15

Amyloid Fibril Polymorphism: Almost Identical on the Atomic Level, Mesoscopically Very Different

Seuring, C., Verasdonck, J., Ringler, P., Cadalbert, R., Stahlberg, H., Böckmann,A., Meier, B.H., Riek, R.

J. Phys. Chem. 121(8), 1783-1792 (2017)

PubMed

DOI: 10.1021/acs.jpcb.6b10624

14

Characterization of mAb dimers reveals predominant dimer forms common in therapeutic mAbs

Plath, F., Ringler, P., Graff, A., Stahlberg, H., Lauer, M., Rufer, A., Graewert, M., Svergun, D., Kellermann, G., Winkler, C., Stücke, J.O., Koulov, A., Schnaible, V.

mAbs 8(5), 928-940 (2016)

PubMed

DOI:

12

Cholesteryl ester transfer between lipoproteins does not require the formation of a ternary tunnel complex with the cholesteryl ester transfer protein

Lauer, M.E., Graff, A., Rufer, A.C., Maugeais, C., von der Mark, E., Matile, H., D'Arcy, B., Magg, C., Ringler, P., Müller, S., Scherer, S., Dernick, G., Thoma, R., Hennig, M., Niesor, E.J., Stahlberg, H.

J. Struct. Biol. 194(2), 191-198 (2016)

DOI: 10.1016/j.jsb.2016.02.016

11

openBEB: open biological experiment browser for correlative measurements

Ramakrishnan, C., Bieri, A., Sauter, N., Roizard, S., Ringler, P., Müller, S., Goldie, K., Enimanev, K., Stahlberg, H., Rinn, B., Braun, T.

BMC Bioinformatics 15, 84 (2014)

PubMed

8

Structural and mechanistic paradigm of leptin receptor activation revealed by complexes with wild-type and antagonist leptins

Moharana, K., Zabeau, L., Peelman, F., Ringler, P., Stahlberg, H., Tavernier, J., Savvides, S. N.

Structure 22(6), 866-77 (2014)

PubMed

7

Review: Cryo-electron microscopy of membrane proteins

Goldie, K., Abeyrathne, P., Kebbel, F., Chami, M., Ringler, P., Stahlberg, H.

In: Kuo J. (eds) Electron Microscopy. Methods in Molecular Biology (Methods and Protocols) Vol. 1117(Humana Press, Totowa, NJ, USA), 325-341 (2014)

PubMed

DOI: DOI

6

Connecting micro-fluidics to electron microscopy

Kemmerling, Simon, Ziegler, J., Schweighauser, G., Arnold, S., Giss, D., Müller, S., Ringler, P., Goldie, K., Goedecke, N., Hierlemann, A., Stahlberg, H., Engel, A., Braun, T.

J Struct Biol 177(1), 128-134 (2012)

PubMed

4

The aquaporin sidedness revisited

Scheuring, S., Tittmann, P., Stahlberg, H., Ringler, P., Borgnia, M., Agre, P., Gross, H., Engel, A.

J. Mol. Biol. 299(5), 1271-1278 (2000)

PubMed

3

Structure of the water channel AqpZ from Escherichia coli revealed by electron crystallography

Ringler, P., Borgnia, M. J., Stahlberg, H., Maloney, P. C., Agre, P., Engel, A.

J Mol Biol 291(5), 1181-90 (1999)

PubMed

2

High resolution AFM topographs of the Escherichia coli water channel aquaporin Z

Scheuring, S., Ringler, P., Borgnia, M., Stahlberg, H., Muller, D. J., Agre, P., Engel, A.

EMBO J 18(18), 4981-7 (1999)

PubMed

1

2009-2021, Senior Scientist

+41 61 387 32 14

C-CINA, Biozentrum, Uni Basel, Mattenstrasse 26, Room P20, CH-4058 Basel, Switzerland

philippe.ringler@unibas.ch