Mohamed Chami

Current Project

BioEm Lab

    Short Biography

    Mohamed Chami from Marocco has been senior scientist in the Stahlberg group, CINA since 2009. He is specialized in high-resolution electron microscopy. His Post Doc experience focused on the crystallization of membrane proteins and was gained at the Biozentrum, University of Basel, Switzerland and the Institut Curie, Paris, France.



    Insight into Lipopolysaccharide Translocation by Cryo-EM structures of a LptDE Transporter in Complex with Pro-Macrobodies

    Botte, M., Ni, D., Schenck, S., Zimmermann, I., Chami, M., Bocquet, N., Egloff, P., Bucher, D., Trabuco, M., Cheng, R.K.Y., Brunner, J.D., Seeger, M.A., Stahlberg, H., Hennig, M. 2021.03.23.436624, (2021)

    DOI: bioRxiv


    Functional and structural characterization of Serratia marcescens ExbB: determinants of the interaction with HasB/TonB

    Biou, V., Adaixo, R., Chami, M., Coureux. P., Laurent B., Ntsogo, Y., Cardoso de Amorim, G., Izadi-Pruneyre, N., Malosse, C., Chamot-Rooke, J., Stahlberg, H., Delepelaire, P. 2021.04.21.440815, (2021)

    DOI: bioRxiv


    Cryo-EM structural studies of the agonist complexed human TRPV4 ion-channel reveals novel structural rearrangements resulting in an open-conformation

    Botte, M., Ulrich, A.K.C., Adaixo, R., Gnutt, D., Brockmann, A., Bucher, D., Chami, M., Bocquet, N., Ebbinghaus-Kintscher, U., Puetter, V., Becker, A., Egner, U., Stahlberg, H., Hennig, M., Holton, S.J.

    bioRxiv , 2020.10.13.334797 (2020)

    DOI: DOI


    Structural basis of Focal Adhesion Kinase activation on lipid membranes

    Acebrón, I., Righetto, R., Schoenherr, C., de Buhr, S., Redondo, P., Culley, J., Rodríguez, C.F., Daday, C., Biyani, N., Llorca, O., Byron, A., Chami, M., Gräter. F., Boskovic J., Frame, M.C., Stahlberg, H., Lietha, D.

    EMBO J. 39(e104743), (2020)

    DOI: DOI


    Self-assembly of a designed nucleoprotein architecture through multimodal interactions

    Subramanian, R.H., Smith, S.J., Alberstein, R.G., Bailey, J.B., Zhang, L., Cardone, G., Suominen, L., Chami, M., Stahlberg, H., Baker, T.S., Tezcan, F.A.

    ACS Central Science 4(11), 1578-1586 (2018)


    DOI: DOI


    Structural basis for regulation of human acetyl-CoA carboxylase

    Hunkeler, M., Hagmann, A., Stuttfeld, E., Chami, M., Guri, Y., Stahlberg, H., Maier, T.

    Nature 558, 470-474 (2018)


    DOI: DOI


    Cryo-EM analysis of homodimeric full-length LRRK2 and LRRK1 protein complexes

    Sejwal, K., Chami, M., Remigy, H., Vancraenenbroeck, R., Sibran, W., Sütterlin, R., Baumgartner, P., McLeod, R., Chartier-Harlin. M.-C., Baekelandt, V., Stahlberg, H., Taymans, J.-M.

    Scientific Reports 7(1), 8667-8678 (2017)


    DOI: DOI


    Prepore Stability Controls Productive Folding of the BAM-independent Multimeric Outer Membrane Secretin PulD.

    Guilvout I., Brier S., Chami, M., Hourdel V., Francetic O., Pugsley A.P., Chamot-Rooke J., Huysmans G.H.

    J. Biol. Chem. 292(1), 328-338 (2017)


    DOI: 10.1074/jbc.M116.759498


    Two-dimensional crystallization of the mouse serotonin 5-HT3A receptor

    Rheinberger, J., Hassaine, G., Chami, M., Stahlberg, H., Vogel, H., Li, X.

    Micron 90, 19-24 (2017)


    DOI: j.micron.2016.10.004


    The lipidome associated with the gamma-secretase complex is required for its integrity and activity.

    Ayciriex, S., Gerber, H., Osuna, G.M., Chami, M., Stahlberg, H., Shevchenko, A., Fraering, P.C.

    Biochemical Journal 473(3), 321-334 (2016)


    DOI: 10.1042/BJ20150448


    Glasslike Membrane Protein Diffusion in a Crowded Membrane.

    Munguira I., Casuso I., Takahashi H., Rico F., Miyagi A., Chami, M., Scheuring S.

    ACS Nano 10(2), 2584-90 (2016)


    DOI: 10.1021/acsnano.5b07595


    Structure and assembly of the mouse ASC inflammasome by combined NMR spectroscopy and cryo-electron microscopy

    Sborgi, L., Ravotti, F., Dandey, V. P., Dick, M. S., Mazur, A., Reckel, S., Chami, M., Scherer, S., Huber, M., Bockmann, A., Egelman, E. H., Stahlberg, H., Broz, P., Meier, B. H., Hiller, S.

    Proc Natl Acad Sci U S A 112(43), 13237-42 (2015)



    Review: Cryo-electron microscopy of membrane proteins

    Goldie, K., Abeyrathne, P., Kebbel, F., Chami, M., Ringler, P., Stahlberg, H.

    In: Kuo J. (eds) Electron Microscopy. Methods in Molecular Biology (Methods and Protocols) Vol. 1117(Humana Press, Totowa, NJ, USA), 325-341 (2014)


    DOI: DOI


    Ligand-induced structural changes in the cyclic nucleotide-modulated potassium channel MloK1

    Kowal, J., Chami, M., Baumgartner, P., Arheit, M., Chiu, P. L., Rangl, M., Scheuring, S., Schroder, G. F., Nimigean, C. M., Stahlberg, H.

    Nat Commun 5, 3106 (2014)



    Molecular assembly of the aerolysin pore reveals a swirling membrane-insertion mechanism

    Degiacomi, M. T., Iacovache, I., Pernot, L., Chami, M., Kudryashev, M., Stahlberg, H., van der Goot, F. G., Dal Peraro, M.

    Nat Chem Biol 9(10), 623-9 (2013)



    Electron microscopy analysis of 2D crystals of membrane proteins

    Abeyrathne, P.D., Arheit, M., Kebbel, F., Castano-Diez, D., Goldie, K., Chami, M., Renault, L., Kühlbrandt, W., Stahlberg, H.

    Comprehensive Biophysics 1, 277-310 (2012)

    DOI: 10.1016/B978-0-12-374920-8.00119-3


    Characterization of the motion of membrane proteins using high-speed atomic force microscopy

    Casuso, I., Khao, J., Chami, M., Paul-Gilloteaux, P., Husain, M., Duneau, J. P., Stahlberg, H., Sturgis, J. N., Scheuring, S.

    Nat Nanotechnol 7(8), 525-9 (2012)



    Double hexameric ring assembly of the type III protein translocase ATPase HrcN

    Müller, S., Pozidis, C., Stone, R., Meesters, C., Chami, M., Engel, A., Economou, A., Stahlberg, H.

    Mol Microbiol 61(1), 119-25 (2006)



    Charting and unzipping the surface layer of Corynebacterium glutamicum with the atomic force microscope

    Scheuring, S., Stahlberg, H., Chami, M., Houssin, C., Rigaud, J. L., Engel, A.

    Mol Microbiol 44(3), 675-84 (2002)



    Head Facility, project management

    +41 61 387 32 24

    C-CINA, Biozentrum, Uni Basel, Mattenstrasse 26, Room P30, CH-4058 Basel, Switzerland