Kenneth Goldie

Research

Broadly based on the Structural analysis of proteins and macro molecular complexes by transmission electron microscopy (TEM) to gain insights into biological processes, function and their relation to disease.

    Short Biography

    Ken Goldie from New Zealand holding dual Swiss Nationality is a research associate and the Facility Manager in the Stahlberg group, C-CINA of the Biozentrum since 2009. Has worked in the Electron Microscopy field since 1980, specialising in high-resolution transmission electron microscopy and scanning EM. Attained a PhD in Structural Biology from The University of Auckland New Zealand and has held positions in 3 New Zealand Universities, Cancer Research UK London, EMBL Heidelberg, and the University of Melbourne Australia. Responsible for the day-to-day running of the microscope facilities in C-CINA and the instruction of new researchers and lab visitors

    Publications

    -+

    Identification of a Dps contamination in Mitomycin-C-induced expression of Colicin Ia

    Pipercevic, J., Jakob, R.P., Righetto, R., Goldie, K., Stahlberg, H., Maier, T., Hiller, S.

    Biochim. Biophys. Acta Biomembr. 1863(7), 183607 (2021)

    PubMed

    DOI: BBAB

    26

    Structural basis of the activation of the CC chemokine receptor 5 by a chemokine agonist

    Tsai, C.-J., Dietz, N.B., Pamula, F., Grahl, A., Goldie, K., Guixa-Gonzalez, R., Schertler, G.F.X., Hartley, O., Stahlberg, H., Maier, T., Deupi, X., Grzesiek, S.

    bioRxiv 2020.11.27.401117, (2020)

    25

    Architecture of the centriole cartwheel-containing region revealed by cryo-electron tomography

    Klena, N., Le Guennec, M., Tassin, A.M., Van den Hoek, H., Erdmann, P.S., Schaffer, M., Geimer, S., Aeschlimann. G., Kovacik, L., Goldie, K., Sadian, Y., Stahlberg, H., Engel, B.D., Hamel, V., Guichard, P.

    EMBO J. e106246, 1-7 (2020)

    PubMed

    DOI: DOI

    24

    A helical inner scaffold provides a structural basis for centriole cohesion

    Le Guennec, M., Klena, N., Gambarotto, D., Laporte, M.H., Tassin, A.M., van den Hoek, H., Erdmann, P.S., Schaffer, M., Kovacik, L., Borgers, S., Goldie, K., Stahlberg, H., Bornens, M., Azimzadeh, J., Engel, B.D., Hamel, V., Guichard, P.

    Science Advances 6(7), eaaz4137 (2020)

    PubMed

    DOI: DOI

    23

    Lewy pathology in Parkinson's disease consists of crowded organelles and lipid membranes

    Shahmoradian, S., Lewis, A., Genoud, C., Graff, A., Pérez Navarro, P., Goldie, K., Sütterlin, R., Castano-Diez, D., Ingrassia, A., Rozemuller, A.J.M., Paepe, A.D., Erny, J.Staempfli, A., Hoernschemeyer, J., Niedieker, D., El-Mashtoly, S.F., Gerwert, K., Bohrmann, B., Britschgi, M., Stahlberg, H., van de Berg, W.D.J., Lauer, M.E.

    Nature Neuroscience 22, 1099-1109 (2019)

    PubMed

    DOI: Nature Neurosc.

    22

    Cryo-EM structures of the pore-forming A subunit from the Yersinia entomophaga ABC toxin

    Piper, S., Brillault, L., Rothnagel, R., Croll, T., Box, J., Chassagnon, I., Scherer, S., Goldie, K., Jones, S., Schepers, F., Hartley-Tassell, L., Ve, T., Busby, J., Dalziel, J., Lott, S., Hankamer, B., Stahlberg, H., Hurst, M., Landsberg, M

    Nature Communications 10(1), 1952 (2019)

    PubMed

    DOI: DOI

    21

    Deuterium induces a distinctive Escherichia coli proteome that correlates with the reduction in growth rate.

    Opitz, C., Ahrne, E., Goldie, K., Schmidt, A., Grzesiek, S

    J. Biol. Chem. 294(7), 2279-2292 (2019)

    PubMed

    DOI: DOI

    20

    Rapid Structure Determination of Microcrystalline Molecular Compounds Using Electron Diffraction.

    Gruene, T., Wennmacher, JTC, Zaubitzer, C, Holstein, JJ, Heidler, J, Fecteau-LeFebvre, A., De Carlo, S, Mueller, E, Goldie, K., Regeni, I, Li, T, Santiso-Quinones, G, Steinfeld, G, Handschin, S, , ., van Bokhoven, JA, Clever, GH, Pantelic, R

    Angew. Chem. Int. Ed. Engl. 57(50), 16313-16317 (2018)

    PubMed

    DOI: DOI

    19

    Phospholipid-block copolymer hybrid vesicles with lysosomal escape ability

    Zong, W., Thingholm, B., Itel, F., Schattling, P.S., Brodszkij, E., Mayer, D., Stenger, S., Goldie, K., Han, X., Städler, B.

    Langmuir 34(23), 6874-6886 (2018)

    PubMed

    DOI: DOI

    18

    3D correlative electron microscopy reveals continuity of Brucella-containing vacuoles with the endoplasmic reticulum

    Sedzicki, J., Tschon, T., Huey-Low, S., Willemart, K., Goldie, K., Lewtesson, J.-J., Stahlberg, H., Dehio, C.

    J. of Cell Science 131(4), (2018)

    PubMed

    DOI: doi

    16

    Cryo-EM reconstruction of Type VI secretion system baseplate and sheath distal end

    Nazarov, S., Schneider, J.PO., Brackmann, M., Goldie, K., Stahlberg, H., Basler, M.

    EMBO Journal 37, e97103 (2018)

    PubMed

    DOI: DOI

    15

    CryoEM structure of the extended type VI secretion system sheath–tube complex

    Wang, J., Brackmann, M., Castano-Diez, D., Kudryashev, M., Goldie, K., Maier, T., Stahlberg, H., Basler, M.

    Nature Microbiology 2(11), 1507-1512 (2017)

    PubMed

    DOI: DOI

    13

    Atomic structure of granulin determined from native nanocrystalline granulovirus using an X-ray free-electron laser

    Gati, C., Oberthuer, D., Yefanov, O., Bunker, R.D., Stellato, F., Chiu, E., Yeh, S.M., Aquila, A., Basu, S., Bean, R., Beyerlein, K.R., Botha, S., Boutet, S., DePonte, D.P., Doak, R.B., Fromme, R., ..., Goldie, K., ..., Barty, A., Chapman, H.N.

    Proc Natl Acad Sci U S A 114(9), 2247-2252 (2017)

    PubMed

    DOI:

    12

    Cell-free reconstitution reveals centriole cartwheel assembly mechanisms

    Guichard, P., Hamel, V., Le Quennec, M., Banterle, N., Iacovache, I., Nemcikova, V., Flückiger, I., Goldie, K., Stahlberg, H., Levy, D., Zuber, B., Gönczy, P.

    Nature Communications 8:14813, (2017)

    PubMed

    DOI:

    10

    Structure of the Full-length VEGFR-1 Extracellular Domain in Complex with VEGF-A

    Markovic-Mueller, S, Stuttfeld, E., Asthana, M., Weiner, T., Bliven, S., Goldie, K., Kisko, K., Capitani, G., Ballmer-Hofer, K.

    Structure 25(2), 341-352 (2017)

    DOI: 10.1016/j.str.2016.12.012

    9

    Cullin-RING ubiquitin E3 ligase regulation by the COP9 signalosome

    Cavadini, S., Fischer, E.S., Goldie, K., Boehm, K., Lingaraju, G.M., Jones, M., Bunker, R.D., Richkule, R., Pantelic, R., Mohamed, W.I., Hassiepen, U., Beckwith, R.E.J., Stahlberg, H., and Thomae, N.H.

    Nature 531(7596), 598-603 (2016)

    PubMed

    DOI: 10.1038/nature17416

    7

    Atomic structure of bacteriophage T4 baseplate and its function in triggering sheath contraction

    Taylor, N., Prokhorov, N.S., Guerrero-Ferreira, R.C., Shneider, M.M., Browning, C., Goldie, K., Stahlberg, H., Leiman, P.G.

    Nature 533(7603), 346-352 (2016)

    PubMed

    DOI: 10.1038/nature17971

    6

    openBEB: open biological experiment browser for correlative measurements

    Ramakrishnan, C., Bieri, A., Sauter, N., Roizard, S., Ringler, P., Müller, S., Goldie, K., Enimanev, K., Stahlberg, H., Rinn, B., Braun, T.

    BMC Bioinformatics 15, 84 (2014)

    PubMed

    5

    Review: Cryo-electron microscopy of membrane proteins

    Goldie, K., Abeyrathne, P., Kebbel, F., Chami, M., Ringler, P., Stahlberg, H.

    In: Kuo J. (eds) Electron Microscopy. Methods in Molecular Biology (Methods and Protocols) Vol. 1117(Humana Press, Totowa, NJ, USA), 325-341 (2014)

    PubMed

    DOI: DOI

    4

    Electron microscopy analysis of 2D crystals of membrane proteins

    Abeyrathne, P.D., Arheit, M., Kebbel, F., Castano-Diez, D., Goldie, K., Chami, M., Renault, L., Kühlbrandt, W., Stahlberg, H.

    Comprehensive Biophysics 1, 277-310 (2012)

    DOI: 10.1016/B978-0-12-374920-8.00119-3

    3

    Connecting micro-fluidics to electron microscopy

    Kemmerling, Simon, Ziegler, J., Schweighauser, G., Arnold, S., Giss, D., Müller, S., Ringler, P., Goldie, K., Goedecke, N., Hierlemann, A., Stahlberg, H., Engel, A., Braun, T.

    J Struct Biol 177(1), 128-134 (2012)

    PubMed

    2

    Head Facility, technical management

    73218

    C-CINA, Biozentrum, Uni Basel, Mattenstrasse 26, Room P.34, CH-4058 Basel, Switzerland

    k.goldie@unibas.ch